Rapid Purification of Endotoxin-Free RTX Toxins

Cytolytic leukotoxins of the repeat in toxin (RTX) family are large proteins excreted by gram-negative bacterial pathogens Jackets through the type 1 secretion system (T1SS).Due to low yields and poor stability in cultures of the original pathogens, it is useful to purify recombinant fatty-acylated RTX cytolysins from inclusion bodies produced in E

read more

Constructivist Stakian Multicase Study: Methodological Issues Encountered in Cross-Cultural Palliative Care Research

Case study research facilitates the in-depth, real-life exploration of complex phenomena from multiple perspectives.It is a well-established approach to deal with the complexities involved in palliative care research.Case studies are not aligned to a single epistemological paradigm but are defined by the identification of the case to be studied.Thi

read more